• Acute chagas’ disease in western Venezuela: A clinical, seroparasitologic, and epidemiologic study 

      Carrasco Guerra, Hugo A.; Parada F., Henry; Crisante R., Gladys E.; Rojas E., Agustina del V.; González V., Nestor E.; Ramirez, José Luis; Guevara, Palmira; Rivero, Christian; Borges P., Rafael E.; Scorza, José Vicente; Añez Reverol, Nestor Oswaldo (The American Society of Tropical Medicine and Hygiene, 1999-02)
      A clinical, parasitologic, and serologic study carried out between 1988 and 1996 on 59 acute-phase patients in areas of western Venezuela where Chagas’ disease is endemic showed 19 symptomatic patterns or groups of symptoms ...
    • La cara oculta de la enfermedad de Chagas en Venezuela 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Rojas E., Agustina del V.; Díaz P., Néstor A.; Añez Rojas, Néstor; Carrasco Guerra, Hugo A.; Parada F., Henry; Aguilera, Marcos; Moreno, Glenda; Galíndez Girón, Itamar; Sandoval, Raquel; Sandoval Godoy, Irene Margarita; Vásquez Paredes, Libia; Nava Rulo, Oscar; Guerra, Francis; Uzcátegui, Gerardo; Yépez, José Yancarlos; Rodriguez, Claudina; Bonfante Cabarcas, Rafael (Boletín de malariología y salud ambiental. Vol. XLII, Nº 2, agosto-diciembre, 2003, 2003-12)
      Se presenta un estudio multidisciplinario y multicéntrico llevado a cabo durante el período 1988 - 2003 por investigadores de 10 instituciones venezolanas con la finalidad de aportar nuevos datos sobre la situación ...
    • Chagas disease inapparent infection in asymptomatic individuals from a Yukpa ethnic community in western Venezuela 

      Añez Reverol, Nestor Oswaldo; Atencio, Ricardo; Rivero, Zulbey; Bracho, Angela; Rojas E., Agustina del V.; Romero, Maximiliano; Crisante R., Gladys E. (2011-12-13)
      Inapparent infections by Trypanosoma cruzi were detected, for the first time, in symptomless seropositive healthy individuals from a Yukpa ethnic community in western Venezuela where Chagas disease had not been previously ...
    • Comparative phylogeography of Trypanosoma rangeli and Rhodnius (Hemiptera: Reduviidae) supports a long coexistence of parasite lineages and their sympatric vectors 

      Da Silva, Flávia Maia; Junqueira, A. C.; Campaner, A.; Rodrigues, A. C.; Crisante R., Gladys E.; Teixeira, Marta M.G.; Ramirez, José Luis; Caballero, C. E.; Monteiro, F. A.; Coura, J. R.; Añez Reverol, Nestor Oswaldo (Molecular Ecology (2007) 16, 2007)
      To make reliable interpretations about evolutionary relationships between Trypanosoma rangeli lineages and their insect vectors (triatomine bugs of the genus Rhodnius ) and, thus, about the determinant factors of lineage ...
    • Detección de infecciones subclínicas por Trypanosoma vivax en bovinos de fincas ganaderas de Mérida, Venezuela 

      Añez Reverol, Nestor Oswaldo; García Lugo, Pablo J.; Crisante R., Gladys E.; Rojas E., Agustina del V.; Bolívar Sánchez, Ana María (Artículo publicado en el Boletín de malariología y salud ambiental. Vol. XLVI, Nº 1, enero-julio, 2006, 2006-07)
      Se registra la detección de infecciones subclínicas por Trypanosoma vivax en bovinos asintomáticos de fincas ganaderas de Mérida, Venezuela. El diagnóstico fue realizado utilizando métodos parasitológicos (examen microscópico ...
    • Detection and significance of inapparent infection in chagas disease in western Venezuela 

      Añez Reverol, Nestor Oswaldo; Rojas E., Agustina del V.; Crisante R., Gladys E.; Carrasco Guerra, Hugo A.; Parada F., Henry; Yépez, José Yancarlos; Borges P., Rafael E.; Guevara, Palmira; Ramirez, José Luis (Am. J. Trop. Med. Hyg., 65(3), 2001, 2001)
      Inapparent infections of Trypanosoma cruzi were detected in symptomless seropositive people living in close proximity, and under the same conditions of risk, to patients with acute Chagas disease. Similar infections were ...
    • Detection of amastigote-like forms in the valve of phlebotomus papatasi Infected with Leishmania major 

      Añez Reverol, Nestor Oswaldo; Tang, Yinshan; Rojas E., Agustina del V.; Crisante R., Gladys E.; Killick Kendrick, Mireille; Killick Kendrick, Robert (Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 98(4), Brasil, 2003-06)
      A massive and homogeneous amount of amastigote-like forms was detected in the stomodeal valve (SV) and the thoracic mid-gut (TMG) of Leishmania major-infected Phlebotomus papatasi, which received a second blood meal 13 to ...
    • Detection of Leishmania causing visceral Leishmaniasis in the old and new worlds by a polymerase chain reaction assay based on telomeric sequences 

      Chiurillo, Miguel Angel; Sachdeva, Monica; Dole, Vananda S.; Yépez, José Yancarlos; Miliani, Efraim; Vázquez, Laura; Rojas E., Agustina del V.; Crisante R., Gladys E.; Guevara, Palmira; Añez Reverol, Nestor Oswaldo; Madhubala, Rentala; Ramirez, José Luis (Am. J. Trop. Med. Hyg., 65(5), 2001, 2001)
      We present a new polymerase chain reaction assay based on telomeric sequences of Leishmania donovani. When this assay was used in dilutions of purified L. donovani DNA, a strong amplification signal was observed with 1 fg ...
    • Detection of trypanosoma cruzi and trypanosoma rangeli infection by duplex PCR assay based on telomeric sequences 

      Chiurillo, Miguel Angel; Crisante R., Gladys E.; Rojas E., Agustina del V.; Peralta, Andreina; Dias, Manuel; Guevara, Palmira; Añez Reverol, Nestor Oswaldo; Ramirez, José Luis (CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Sept. 2003, 2003-09)
    • Domiciliación de triatoma nigromaculata de la región montana del sur de Mérida, Venezuela 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Rojas E., Agustina del V.; Díaz P., Néstor A.; Diaz, Silverio; Lizano Araque, José Eliézer; Superlano, Ysmar; Aldana Carrasco, Elis José (Boletín de malariología y salud ambiental. Vol. XLV, Nº 1, enero-julio, 2005, 2005-07)
      Se reporta el hallazgo de Triatoma nigromaculata (Stâl, 1859) de la region montañosa de Merida, Venezuela. Los especímenes, 10 adultos y 15 ninfas, fueron colectados dentro de viviendas a 1.750 m snm. Aparentemente, ésta ...
    • Estudio epidemiológico de Leishmaniasis visceral en El Limón, al norte del estado Lara, Venezuela 

      Vargas Díaz, Ersi; Añez Reverol, Nestor Oswaldo; Rojas E., Agustina del V.; Crisante R., Gladys E.; Yépez, José Yancarlos (Boletín de malariología y salud ambiental. Vol. XLIV, Nº 2, agosto-diciembre, 2004, 2004-12)
      El Limón es una comunidad rural ubicada en el Municipio Urdaneta, al Norte del Estado Lara, que limita al Norte con el Municipio Unión,Estado Falcón, Venezuela, una conocida área endémica de leishmaniasis visceral. Debido ...
    • Evaluation of conventional serological tests for the diagnosis of American cutaneous Leishmaniasis 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Rojas E., Agustina del V. (Boletín de malariología y salud ambiental. Vol. XLVII, Nº 1, enero-julio, 2007, 2007-07)
      Three serological tests (ELISA, IFAT, DAT) were evaluated using sera from selected individuals with different American cutaneous leishmaniasis (ACL) clinical conditions. Reactivity in at least 2 of the 3 named tests was ...
    • Expression of fluorescent genes in trypanosoma cruzi and trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its application to parasite-vector biology 

      Guevara, Palmira; Dias, Manuel; Rojas E., Agustina del V.; Crisante R., Gladys E.; Abreu Blanco, María Teresa; Umezawa, Eufrosina S.; Vazquez, Martin; Levin, Mariano J.; Añez Reverol, Nestor Oswaldo; Ramirez, José Luis (Journal of Medical Entomology 42(1), 2005-01)
    • Expression of GP82 and GP90 surface glycoprotein genes of Trypanosoma cruzi during in vivo metacyclogenesis in the insect vector Rhodnius prolixus 

      Cordero, Esteban M.; Gentil, Luciana G.; Crisante R., Gladys E.; Ramirez, José Luis; Yoshida, Nobuko; Añez Reverol, Nestor Oswaldo; da Silveira, José Franco (Acta Tropica 105 (2008), 2008)
      Trypanosoma cruzi, the parasite causing Chagas’ disease, relies on triatomines for its transmission. T. cruzi metacyclic trypomastigotes express GP82 and GP90, which are developmentally regulated surface proteins that have ...
    • Genetic typing of Trypanosoma cruzi isolates from different hosts and geographical areas of western Venezuela 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Añez Rojas, Néstor; Rojas E., Agustina del V.; Moreno, Glenda; Da Silva, Flávia Maia; Teixeira, Marta M.G. (2011-12-13)
      A total of 72 Trypanosoma cruzi isolates from different hosts and geographical regions of western Venezuela, where Chagas disease is endemic, were typed using ribosomal and mini-exon gene markers. The isolates were obtained ...
    • Infección natural por Trypanosoma cruzi en Panstrongylus geniculatus (Latreille, 1811) de la región montana de Mérida, Venezuela 

      Añez Reverol, Nestor Oswaldo; Saavedra, Carlos; Crisante R., Gladys E.; Rojas E., Agustina del V.; Lizano Araque, José Eliézer (Boletín de malariología y salud ambiental. Vol. XLV, Nº 2, agosto-diciembre, 2005, 2005-12)
      Se reseña el hallazgo de un ejemplar de Panstrongylus geniculatus naturalmente infectado con Trypanosoma cruzi en el interior de una vivienda en la localidad de El Morro, Mérida, Venezuela. Se registra por primera vez la ...
    • Infected dogs as a risk factor in the transmission of human Trypanosoma cruzi infection in western Venezuela 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Rojas E., Agustina del V.; Teixeira, Marta M.G. (Acta Tropica 98 (2006), 2006-07)
      A total of 565 mongrel dogs from rural localities of Venezuela were examined by serological (DAT, IFAT and ELISA) and parasitological tests to address the status of Trypanosoma cruzi infection and to evaluate their role ...
    • Isolation, purification, characterization and antigenic evaluation of GPI-anchored membrane proteins from Leishmania (Viannia) braziliensis 

      Rojas E., Agustina del V.; García Lugo, Pablo J.; Crisante R., Gladys E.; Añez Reverol, Nestor Oswaldo; Añez Rojas, Néstor (Acta Tropica 105 (2008), 2008)
      GPI-anchored proteins from the plasma membrane of Leishmania (Viannia) braziliensis promastigotes were isolated, characterized and their migration pattern compared with those from other Leishmania species. In all cases the ...
    • Myocardial parasite persistence in chronic chagasic patients 

      Añez Reverol, Nestor Oswaldo; Carrasco Guerra, Hugo A.; Parada F., Henry; Crisante R., Gladys E.; Rojas E., Agustina del V.; Fuenmayor Meza, Carmen Elena; González V., Nestor E.; Percoco, Gloria; Borges P., Rafael E.; Guevara, Palmira; Ramirez, José Luis (Am. J. Trop. Med. Hyg., 60(5), 1999, 1999)
      The persistence of Trypanosoma cruzi tissue forms was detected in the myocardium of seropositive individuals clinically diagnosed as chronic chagasic patients following endomyocardial biopsies (EMBs) processed by ...
    • Nuevos casos agudos de enfermedad de Chagas en el Occidente de Venezuela 

      Añez Reverol, Nestor Oswaldo; Crisante R., Gladys E.; Parada F., Henry (Salus. Revista de la Facultad de Ciencias de la Salud. Universidad de Carabobo. Diciembre 2007 - vol. 11, Supl. Nº 1, 2007-12)
      Chagas en pacientes procedentes de regiones endémicas de los estados Barinas (7) y Trujillo (2) al occidente de Venezuela, 2 de los cuales (22%) fueron fatales. Se dan detalles clínicos, seroparasitológicos y moleculares ...