Biodiversity of active toxins and proteomic analysis of lansberg´s Mapanare (Porthidium lansbergii hutmanni) Venom snake and its impact on envenoming

Abstract

The venom characterization of Lansberg’s hognose viper (Porthidium lansbergii hutmanni) was described using a combination of SDS-PAGE are 1 and 2d electrophoresis, MALDI TOF/TOF and tandem mass spectrometry (LC-MS / MS), as well as some proteolytic activities. This study shown the existence of metallo and serine proteases, cysteine-rich proteins (CRISPs), and L-amino acid oxidases (LAAOs), which appeared in variable amounts. It was also described, for the first time in this venom, the presence of the glutaminil-cyclase (vQC) that had been only before observed in species of Crotalus atrox, C. godmani, C. d. terrificus and M. fulvius of the American continent, but not in Porthidium genus. The presence of toxins with a gelatinolytic and caseinolytic activity was also defined. The crude venoms are excellent sources of enzymatic and non-enzymatic actions, which should be purified and characterized with the aim of their possible use as therapeutic agents, in antitumor and haemostatic treatments.