Comparative phylogeography of Trypanosoma rangeli and Rhodnius (Hemiptera: Reduviidae) supports a long coexistence of parasite lineages and their sympatric vectors
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Date
2007Author
Palabras Clave
Evolution, Phylogeography, Rhodnius, Ribosomal gene, Spliced-leader gene, Trypanosoma rangeliMetadata
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To make reliable interpretations about evolutionary relationships between
Trypanosoma rangeli lineages and their insect vectors (triatomine bugs of the genus
Rhodnius ) and, thus, about the determinant factors of lineage segregation within
T. rangeli , we compared phylogenies of parasite isolates and vector species. Sixty-one T. rangeli isolates from invertebrate and vertebrate hosts were initially evaluated in terms of polymorphism of the spliced-leader gene (SL). Further analysis based on SL and SSUrRNA sequences from 33 selected isolates, representative of the overall phylogenetic diversity and geographical range of T. rangeli, supported four phylogenetic lineages within this species. By comparing the phylogeny of
Rhodnius species with that inferred for
T. rangeli isolates and through analysis of the geographical range of the isolates, we showed that there is a very significant
overlap in the distribution of Rhodnius
species and T. rangeli lineages. Congruence between phylogeographical analysis of both
T. rangeli lineages and complexes of Rhodnius
species are consistent with the hypothesis of a long coexistence of parasites and their vectors, with lineage divergence associated with sympatric species of Rhodnius apparently without association with particular vertebrate hosts. Separation of
T. rangeli isolates from vectors of
distinct complexes living in sympatry favours the absence of gene flow between the lineages and suggests evolution of T. rangeli
lineages in independent transmission cycles, probably associated to specific Rhodnius
spp. ecotopes. A polymerase chain reaction assay based on SL intergenic sequences was developed for simultaneous identification and lineage genotyping of T. rangeli in epidemiological surveys.
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Información Adicional
Correo Electrónico | gecr68@hotmail.com jramirez@reacciun.ve nanes@ula.ve |
Editor | Molecular Ecology (2007) 16 |
Colación | 3361–3373 |